›› 2009, Vol. 40 ›› Issue (3): 419-422.doi: 10.3969/j.issn.0529-1356.2009.03.015

• 论著 • 上一篇    下一篇

不同浓度内皮素对体外人心包间皮细胞内钙离子浓度的影响

刘洁琳;温绍君*;赵莉敏;王佐广;文杰;刘雅 ;刘亚萍; 陈新军   

  1. 首都医科大学附属北京安贞医院北京市心肺血管疾病研究所高血压研究室,北京 100029
  • 收稿日期:2008-03-05 修回日期:2008-05-14 出版日期:2009-06-06
  • 通讯作者: 温绍君

Effect of different concentrations of endothelin on intracellular calcium level in cultured human pericardial mesothelial cells

  1. Department of Hypertension Research, Beijing Anzhen Hospital of Capital Medical University,Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
  • Received:2008-03-05 Revised:2008-05-14 Online:2009-06-06
  • Contact: WEN Shao-jun

关键词: 心包间皮细胞, 内皮素, 钙离子, 激光扫描共焦显微镜, 人

Abstract: Objective To investigate the effect of different concentrations of endothelin on intracellular calcium level in cultured human pericardial mesothelial cells. Methods Pericardial mesothelial cells from patients undergoing open heart surgery were cultured in vitro. Cells of passage 8-10 were used in this experiment. When confluence was reached, the mesothelial cells were loaded with Fluo3/AM. The intracellular calcium concentrations in theses cultured cells were determined by confocal laser scanning microscopy after 10SUP>-7/SUP>mol/L, 10SUP>-8/SUP>mol/L, 10SUP>-9/SUP>mol/L ET being added respectively. Results Calcium concentrations in cultured mesothelial cells increased remarkably after adding three kinds of ET concentration(EM>P/EM><0.001). There was interaction between endothelin concentration and observation time (P<0.001). Conclusion Calcium in cultured mesothelial cells changed distinctly after the stimulation of different concentration of ET; Human pericardial mesothelial cells may possess important biological activity and endothelin may play an important role in the pericardium in an autocrine and/or paracrin

Key words: Pericardial mesothelial cell, Endothelin, Calcium, Confocal laser scanning microscope, Humen

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